HCC827 barcode libraries in vitro and in vivo . (a) Diagram of HCC827 passaging experiments. (b) Clones were counted and binned in Log 2 bins based on percentage (frequency) within the population, from least to greatest. The percentage of clones in each bin is shown. Inset shows magnification of larger bins. HCC827 biological replicate A and tumor 1 are shown (others are shown in Additional files 14 (in vitro) and 15 (tumors)). (c) The percentage of clones, ranked from most to least frequent, plotted by what percentage of the population they made up. (d) The percentage of the population made up by the top indicated percentages of clones for each sample. (e) The number of clones found in each sample. (f) Rank order clones by percentage of the population for each sample; greatest to least. Any clones ≥1% are shown as white sections, the remaining population of clones smaller than 1% are represented by black in each column. The same barcode occurring as a major clone in more than one sample is marked by color. Stars in PD 0 column indicate positions of yellow, green, and blue clones in that sample. Days post-PD 0 are listed beneath each sample.