K562 cellular barcode libraries. (a) Workflow from plasmid barcode library to cellular barcode library. Unique barcodes are represented as different colored rectangles; barcoded cells also express eGFP. (b) Experimental design of passaging experiments. (c) Clones were counted and binned in Log 2 bins based on percentage (frequency) within the population, from least to greatest. The percentage of the clones in each bin is shown. Inset shows magnification of larger bins. K562 biological replicate A is shown (others are shown in Additional file 2). (d) The percentage of clones, ranked from most to least frequent, plotted by what percentage of the population they made up. (e) The percentage of the population made up by the top indicated percentages of clones for each sample. (f) The number of clones found in each sample. (g) Rank order barcodes by percentage of sequences for each sample; greatest to least. Any clones ≥1% are delimited by white sections within the column, while the remaining population of clones smaller than 1% are represented by the black area in each column. The same clone occurring as a major clone in more than one sample is identified by color.