Barcode plasmid library analysis. Results from four separate PCR amplification and sequencing runs of the plasmid barcode library (A to D). (a) The number of barcodes found in each replicate after analysis and trimming. 'Mean' is the average number of barcodes for the four replicates; 'Total' is the number of unique barcodes found within the four samples combined. (b) Venn diagram demonstrating the amount of overlap of barcodes among the four replicates. Darker shading indicates larger numbers of barcodes. (c) Barcodes were counted and grouped in Log 2 bins based on percentage (frequency) within the population, from least to greatest. The percentage of the barcodes in each bin is shown. (d) The predicted (Expected) and experimentally determined median and mean barcode frequencies are shown as percentages, as well as the standard deviation from the mean. (e) The percentage of barcodes, ranked from most to least frequent plotted by what percentage of the total sequences they made up. Dashed line represents perfectly equal representation of barcodes. (f) The percentage of sequences made up by the top indicated percentages of the barcodes for each sample.