Validation of SoxN direct targets. (A) Selected genes from a hierarchical clustering of expression trends highlighting three functional groups of SoxN direct targets. logFC = log2 fold change. (B) Immunohistochemical stainings of a selection of SoxN direct targets identified in this study in control embryos (left), SoxN homozygous mutants (middle) and Kr-Gal4 UAS-SoxN embryos (right). All embryos are shown as ventral views with anterior to the left. Ind, showing normal expression in mutant and transgenic embryos, is provided as a negative control.