Figure 1

SoxN functional studies. (A) Overview of the SoxN datasets generated. Each coloured box below the time line represents a single gene expression, DNA adenine methyltransferase identification (DamID) or chromatin immunoprecipitation (ChIP) experiment performed at the indicated time ranges. Above the time line, major events in neural development are indicated and illustrated with FlyBase images adapted from Volker Hartenstein, Atlas of Drosophila Development, Cold Spring Harbor Laboratory Press, 1993. (B) Partitioning of genes differentially expressed in SoxN mutants. Probes corresponding to differentially expressed genes were divided into three groups (downregulated, upregulated and variable) according to their expression trend over time. (C) Genomic profiles of SoxN binding. Window scores and binding intervals at a false discovery rate (FDR) of 1% and FDR of 25% are displayed for the SoxNDam dataset (purple), and binding intervals at FDR 25% are shown for the SoxNPA179 Early (red), SoxPA179 Late (orange), SoxND1 (blue) and SoxND2 (green) ChIP datasets. SoxN core binding intervals are displayed in black, matches to the SoxN binding motif as thin bars and the locations of know cis-regulatory modules (CRMs) as grey bars. SoxN binding in the gsb-n and gsb (top panel), nerfin-1 (middle panel) and pdm2 (bottom panel) regions are displayed.