Reproducibility of methylation levels. (A) Differentially methylated cytosines identified in RRBS sequencing data derived from technical replicates, biological replicates, and samples from different mouse strains in B6 and DBA female or male mice. Error bars represent the standard deviation. (B,C) Distribution of the variance in methylation levels. The plots show the empirical cumulative distribution function (CDF) for the variance in methylation levels for all cytosines (B) and for CpGs (C). The distribution of biological replicates is shown by the blue curve, of technical replicates by the black curve, and of different mouse strains by the red curve.