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Figure 3 | Genome Biology

Figure 3

From: Proof by synthesis of Tobacco mosaic virus

Figure 3

Encapsidation and infectivity of synthetic 1-34CE-30 and 1-34CE-ToMV CP-30. (A) 1-34CE-30 RNA encapsidated in vitro with FL-TMV-NA CP (stained with uranyl acetate and imaged by transmission electron microscopy; distance between first and last black ticks in white bar = 200 nm). (B) FL-TMV-NA CP disks prior to the addition of 1-34CE-30 RNA (stained with uranyl acetate and imaged by transmission electron microscopy; distance between first and last black ticks in white bar = 100 nm). (C) Mosaic symptoms on N. tabacum cv. Xanthi SX plant inoculated with encapsidated 1-34CE-30 (4 weeks after inoculation). (D) N. tabacum cv. Xanthi NN local lesions from 1-34CE-30 are to the left of the midrib and local lesions from 1-34CE-ToMV CP-30 are to the right. (E) 1-34CE-ToMV CP-30 virion from Xanthi SX (stained with uranyl acetate and imaged by transmission electron microscopy; distance between first and last black ticks in white bar = 200 nm). (F) N. sylvestris infected with 1-34CE-ToMV CP-30.

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