The functional enrichment of the 29 signature clusters and identification of significant chromatin modification regions for an example cluster. (A,B) Each row represents a cluster and each column represents a functional term or canonical pathway. The color of each square represents the statistical significance of enrichment. The enriched terms are ranked by co-occurrence and only the top 10 are shown. (A) Biological functions. (B) Canonical pathways. N.S., not significant. (C) The chromatin modification heatmap for cluster 9. Each column represents a transcript and each row represents a chromatin mark at a specific genomic region. Only 10 example transcripts are shown here. Each square represents the chromatin change in log2 scale. The transcriptional change is simply indicated as a binary value. The significantly modified regions are marked by asterisks to the right of their names. An example transcript, ENSMUST00000141539 of gene Dvl1 (a protein in the WNT/β-catenin pathway), is further illustrated in (D-F). (D) Genome browser screenshot for the demo transcript with H3K4me3 and H3K27me3 in the top and bottom two tracks, respectively. The exon structures of the demo transcript and another transcript of the same gene are shown below the chromatin tracks. The genomic regions are marked by letters ('u', upstream intergenic; 'p', promoter exon; 'c', canonical exon; 'v', variant exon; 'i', intron), and are further followed by numbers to distinguish different regions. (E,F) Mean and standard error of the mean of the significant regions with some neighbors are shown as line plots with error bars. The x-axis represents different regions from 5’ to 3’ and the y-axis represents normalized coverage. (E) H3K4me3 at u, p, i1, i2 and c. (F) H3K27me3 at v1, i3, i4, v2 and i5.