Experimental overview. A custom prototrophic strain is mated to the entire deletion collection and haploids are selected via SGA. The resulting prototrophic deletion collection is plated out onto 28 distinct metabolic media, and time course growth rate data are extracted from plate images. Growth rates are normalized to a glucose:ammonia reference (constructed from six replicates) and z-scores are calculated for each deletion, in each condition (except glycerol). WT, wild-type.