H3.3 presence coincides with RNA Polymerase II distribution. (A) H3.3 enrichment scores of promoter-associated and TTS-associated H3.3-enriched nucleosomes. Horizontal bars indicate median values. (B) Metagene plots across gene bodies (blue bar) were constructed between -3 kb and +3 kb for H3.3 signals normalized to histone signals. Here and in subsequent metagene plots, positions plotted on the x-axis are relative to annotated transcription start site and TTSs and given in kilobases for upstream and downstream sequences and as percentages for gene bodies. Genes were grouped according to the presence of H3.3-enriched nucleosomes in the promoter (red), close to the TTS (green) or both (blue). (C) Immunocytological detection (upper) of H3.3 (left) and RNA Polymerase II (Pol II; center). An overlay is shown at the right. Scale bars: 5 μm. The diagram (lower) shows H3.3 (green) and Pol II (red) signals along a section through the nucleus (yellow line in the overlay image). (D) Metagene plot of Pol II across gene bodies (blue bar) between -3 kb and +3 kb. Pol II ChIP-chip data are from . Genes were grouped according to the presence of H3.3-enriched nucleosomes in the promoter (red), close to the TTS (green) or both (blue).