Figure 4

Contacts throughout the HOXA cluster distinguish fusion subtypes. (A) The information gain scores of HOXA contacts distinguishing MLL-AF9 from MLL-ENL leukemias are represented in heatmap form. The values are color-coded according to the scale shown at the bottom of the heatmap. Numbers on the left and right of heatmap identify BglII restriction fragments corresponding to the restriction pattern shown below the HOXA diagram. Intersecting column and row numbers identify DNA contacts. (B) Averaged interaction frequencies of 20 contacts with high information gain scores (IF values between MLL fusions are statistically different, P <0.01). Interaction frequencies in MLL fusion datasets are the averaged values from Additional file 4: Figure S2. Error bars represent the standard error of the mean. (C) Distribution of informative contacts for classification, and binding of CTCF and cohesin in THP-1 cells along the human HOXA cluster region examined. The y-axis shows the number of CTCF ‘Tags per 10 millions’ obtained by ChIP-seq after normalization against input, across the region characterized (x-axis). CTCF peaks are numbered from left to right (CTCF1 to 7).