Figure 7

Saturation of novel miRNA prediction. To assess the influence of data magnitude on the analyses, saturation curves of the 94 datasets were performed. (a) Saturation curve of sequencing depth, from 10% to 100% of reads retained. For each dataset this percentage of (randomly chosen) reads were retained and subsequently the miRNA prediction analysis was repeated. The total number of reported novel miRNAs (brown) or high-confidence novel miRNAs (orange) is shown. The number of known miRNAs that are detected by simple sequence matches is shown in green. (b) As before, except that entire datasets rather than individual reads were discarded or retained.