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Figure 6 | Genome Biology

Figure 6

From: Evidence for the biogenesis of more than 1,000 novel human microRNAs

Figure 6

Profiling novel miRNAs during differentiation with capture. (a) Saturation curve of miRNA detection with or without SureSelect capture-based enrichment. For sequencing depths ranging from 10 thousand to 30 million reads, the number of known target miRNAs detected is shown. Differentiated neuroblastoma cells were profiled. (b) As before, but for novel miRNAs. (c-f) Profiling depth with or without capture. The histograms show for each target miRNA how many times it is detected. (g) miRNA expression changes measured with or without capture. Neuroblastoma cells were induced to differentiate, and fold-changes were estimated for 140 known miRNAs that could be reliably profiled both with and without using the capture system. (h) miRNA expression changes during differentiation. The capture data from the previous figure could be used to robustly profile 428 known and novel target miRNAs, as cells underwent differentiation to neuron-like state. For each miRNA the normalized expression in the two states is shown. Five miRNAs have previously been observed to be regulated during differentiation of SH-SY5Y cells [42]. Our results are in agreement with the up- or down-regulation of four of these miRNAs, while the fifth, miR-422, is a border case in our measurements.

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