Figure 3

Three novel miRNAs and experimentally identified targets. (a) miRNA candidate 2375 targets Dicer mRNA. The density plot and read alignments show the distribution of sequenced RNAs mapping to the miRNA precursor, summing over 15 sRNA-seq datasets. The two miRNA strands are indicated in light and dark green. Above, the RNA structure of the precursor. Below, the candidate 2375 miRNA (dark green) ligated to the interacting Dicer mRNA (blue). (b) Candidate 153 is integrated in protein biosynthesis pathways. The miRNA is derived from an intron of the EIF2B3 translation factor, and interacts with mRNA of PSGM1, an established chaperone, and FPBP9, involved in protein folding. (c) Candidate 1331 targets ribosomes. It interacts with three mRNAs, of which two are distinct parts of the same 60S ribosomal subunit (RPL8 and RPL13A).