Figure 3

The duplication region of the MDM2 gene on chromosome 12 in samples D-01 T and D-02 T. (a) Mapping depth plots of the two chromosomes. (b) Thin black spikes were read at mapping depth of 2000-base width. The y-axis shows relative depth. Each unit represents approximately 30 times sequencing depth. (c) Gene positions and names around the amplified regions. The black bands show gene locations. (d) MDM2 transcript levels in tumor and adjacent normal tissue paired samples and normal cell lines. Quantitative RT-PCR was used to measure MDM2 mRNA levels in samples D-01 and D-02 (containing amplified MDM2 regions), D-04, D-05, D-10, I-03, and I-04 (without amplified MDM2 regions), and three normal cell lines (HDF, HMEC, and Hs 738.St/Int). Error bars were calculated from two separated experiments of triplicate reactions.