Figure 4

Runx2 binding at known osteogenic genes and a potential target, Ezh2, during osteoblastogenesis. (A) Runx2 binding at three known osteogenic genes, Runx2, Osx, and Ocn, during three stages of osteogenesis: proliferation (day 0); matrix deposition (day 9); and mineralization (day 28). Gene annotation follows standard gene prediction display conventions used by the UCSC genome browser (exons, solid boxes; introns, solid lines; direction of gene transcription, arrows). Positions of Runx2 peaks called by MACS (green bars) and Runx2 consensus motif (TGTGGT; solid black bar) are also depicted. (B) Ezh2 locus bound by Runx2 with increasing Runx2 enrichment over temporal stages of osteogenesis. (C) Ezh2 expression during osteogenesis (relative to day 0 levels) were determined by quantitative RT-PCR and normalized by Hprt1 mRNA levels. Relative mRNA levels are plotted as mean ± SEM from three independent biological replicates.