Correlation of the hmC and mC changes at the exon-intron boundary with gene expression and splicing. (a, b) The hmC changes at the exon-intron boundary (a) and across the exon (b) are similar for exons expressed at high (red), middle (green), or low (blue) levels, as well as exons with no expression (black). (c, d) The percentages of alternatively spliced exons (c) and the inclusion rates (d) of the exons with the status of the 5′ss CpG site being mChigh, hmChigh, modClow, or modCno showing differential splicing of these exon types. Two-tailed MWW test. n, number of exons.