Figure 4

STAiR1 – a continuous specifically expressed transcript. (A) INA6 cells were restimulated with IL-6 as described in Figure 3A and chromatin immunoprecipitated (ChIP-ed) for tri-methylated H3K4 and H3K36, respectively. Enrichment compared to an IgG isotype control was assessed by quantitative real-time PCR using primer sets P1, P3, P5 and P6. The location of respective amplicons is shown in Figure 3A. Strong enrichment for H3K4me3 is observed only within P1, indicating an active promoter region. H3K36me3 shows strong enrichment throughout the STAiR1 transcript. (B) Expression z-score aggregated over STAiR1 expressed after 1 h (STAiR1 short, chr18:41,591,020-41,720,348) or the entire annotated STAiR1 transcript (STAiR1 long). (C) INA6 cells were restimulated with IL-6 as described and induction of STAiR1 was detected using qRT-PCR with primer sets P1 to P6, as shown in Figure 3A, and using GAPDH for normalization. This expression time course is consistent with the time-dependent elongation of STAiR1 observed in the tiling array data shown in Figure 3A. (D) Expression of macroRNAs in different tissues, as detected by reverse transcriptase PCR, using GAPDH as a normalization control. Tissue specificity varies strongly between different macroRNAs. STAiR, STAT3-induced RNA; STAT3, signal transducer and activator of transcription-3.