MOABS detects differential 5hmc using RRBS and oxBS-Seq. (a) Simulation study of 5hmc detection from oxBS-seq and RRBS. Each point on curves represents the smallest number of reads (X-axis) needed to detect a 5hmc ratio (Y-axis) at specified 5mc ratio (indicated as colors). The thin and thick curves represent FETP and MOABS, respectively. (b) Beta distribution of 5hmc ratio in each sample.