Figure 2
From: Whole genome sequencing of Ethiopian highlanders reveals conserved hypoxia tolerance genes
RNAi-mediated knockdown of candidate human gene orthologs enhanced hypoxia tolerance in D. melanogaster. The available UAS-RNAi lines for cic (ortholog of human CIC), Hsl (ortholog of human LIPE) and Paf-AHα (ortholog of human PAFAH1B3) were crossed with the daughterless (da)-GAL4, a driver strain that expresses GAL4 ubiquitously. The level of hypoxia tolerance was determined by measuring eclosion rate in an atmosphere chamber containing 5% O2. The UAS-RNAi stocks without cross were used as a negative control (open bars). Two different UAS-RNAi lines targeting each candidate gene were used in each experiment to minimize off-target effects. Each bar represents the mean ± standard error of the mean value of three separate tests; *P < 0.05.