A single mutation in the GSTe2 gene allows tracking of metabolically based insecticide resistance in a major malaria vector

Table 3 Genetic parameters between coding and non-coding regions for all three genes in the three countries

		Complete sequenced fragment (882 bp)							Coding region (663 bp)							Non-coding region (219 bp)
Gene	2n	S	h	hd	π	θ	D	D*	S	h	hd	π _c	θ	D	D*	S	h	π _nc	D	D*
Benin	24	1	2	0.08	0.09	0.3	−1.15 ns	−1.6 ns	1	2	0.08	0. 13	0.4	−1.15 ns	−1.6 ns	0	1	0	–	–
Cameroon	14	7	8	0.89	2.9	2.5	0.6 ns	0.21 ns	5	6	0.77	2.4	2.4	0.12 ns	−0.22 ns	2	3	4.2	1.3 ns	0.93 ns
Ghana	16	19	10	0.86	5.4	6.8	−0.8 ns	−0.38 ns	4	5	0.67	2.0	1.8	0.35 ns	0.25 ns	15	9	16	−1.1 ns	−0.55 ns
Mozambique	10	12	8	0.95	3.6	5.2	−1.4 ns	−1.4 ns	2	3	0.37	0.6	1.1	−1.4 ns	−1.6 ns	10	8	13	−1.3 ns	−1.25 ns
Malawi	18	14	7	0.78	4.4	4.6	−0.14 ns	1.14 ns	4	5	0.71	1.6	1.7	−0.28 ns	0.21 ns	10	6	13	−0.05 ns	1.41^*
Uganda	10	17	9	0.97	7.5	6.8	0.5 ns	0.15 ns	3	4	0.71	1.7	1.6	−0.76 ns	−1.12 ns	14	7	26	0.72 ns	0.41 ns
Total	92	37	39	0.86	5.1	8.7	−1.3 ns	−0.33 ns	13	16	0.73	2.3	3.8	−1.07 ns	−0.8 ns	24	29	0.013	−1.3 ns	−0.06 ns

2n, number of sequences; D, Tajima’s statistics; D*, Fu and Li’s statistics; h, number of haplotypes; hd, haplotype diversity; ns, not significant; π, nucleotide diversity multiplied by 10³; S, number of polymorphic sites; θ, Watterson’s estimator (per site) multiplied by 10³.

ISSN: 1474-760X