Mutations in emr-1 or lem-2 produce local changes in nuclear organization. (A) Comparison of the regions enriched for Dam::LMN-1 signals in chromosomes I, III and IV in wild type (WT, blue), emr-1(gk119) (black) or lem-2(tm1582) (orange) nematodes (100-kb window MA2C score). (B) Boxplots (as in Figure 1) of Dam::LMN-1 MA2C scores at probe level in LADs, ‘EMR-1 only’ or ‘LMN-1 only’ elements in wild type, emr-1(gk119) or lem-2(tm1582) nematodes. Dam::EMR-1 MA2C scores are included in the middle and right panels to illustrate the mutually exclusive association of ‘EMR-1 only’ and ‘LMN-1 only’ elements with EMR-1 and LMN-1 (***P < 10-11, Wilcoxon rank sum test). (C) Genome browser views of Dam::LMN-1 occupancy in wild type and emr-1(gk119) mutants. (D) Distribution of regions with reduced LMN-1 association in emr-1(gk119) mutants ('Detached regions') in promoters, introns and exons compared to the distribution of the genome. Probability values from one-sided binomial tests are indicated (**P < 10-4, ***P < 10-11).