Figure 1

LMN-1 and EMR-1 have overlapping DNA association profiles devoid of active chromatin marks. (A) Genome-wide correlation plot of LMN-1 and EMR-1 occupancy. The correlation coefficient (r) is at the lower right corner. (B) Regions enriched for Dam::LMN-1 and Dam::EMR-1 in chromosomes V and X. For each track, the average MA2C score probe signal of three independent biological replicates is plotted. Other chromosomes are shown in Figure S3 in Additional file 1. (C) Representative region enriched for LMN-1 (MA2C score; blue) and EMR-1 (MA2C score; green) association. Positions of annotated genes are indicated below. LMN-1 and EMR-1-associated domains (LADs and EADs, respectively; black bars) were defined by a sliding window algorithm. (D) Sizes of LADs, EADs and regions between domains (Gaps). Bottom and top of boxes indicate the 25^th and 75^th percentiles, respectively, and lines in the boxes indicate medians. Whiskers indicate the lowest or the highest data point within 1.5× interquartile range from the box. Probability value from Wilcoxon rank sum test is indicated: **P < 10^-4. (E) Relationship between chromosome size and LMN-1 or EMR-1 relative occupancy (sum of DamID domains divided by chromosome size). Linear regression lines for LMN-1 (blue; r = 0.82) and EMR-1 (green; r = 0.93) occupancy on autosomes are indicated. (F) Average H3K4me3 and H3K36me3 profiles at EAD boundaries. Chromatin immunoprecipitation (ChIP)-chip probe signal scores for indicated histone modifications are plotted. For comparison, averages of LMN-1 and EMR-1 DamID MA2C score are also shown.