Figure 5

Nrd1 and Nab3 binding to a selected number of protein-coding transcripts. (A-G) Shown are UCSC genome browser images for a number of genes predicted to be regulated by Nrd1-Nab3. Coverage of unique cDNAs from Nrd1, Nab3 and Pol II (Rpb2) PAR-CLIP data [6, 29] on Watson (+) and Crick (-) strands is shown as black histograms. Locations of cross-linked Nrd1-Nab3 motifs (this work), annotated Xrn1-sensitive unstable transcripts (XUTs), polyadenylation sites and UTRs [22, 38–41] are included as rectangles. Genomic features located on the Watson (+) strand are indicated in red, whereas features on the Crick (-) strand are indicated in blue. ‘Selected intervals’ indicate genomic regions with a read coverage FDR ≤0.01. These were used for pyMotif analyses.