Figure 8

Sm proteins associate with mature mRNAs. (a) Meta-gene analysis of read density around splice sites for all Drosophila and human Sm-associated intron-containing mRNAs in all RIP-seq experiments. (b) Meta-gene analysis of read density along the gene length for all Drosophila Sm-associated mRNAs quantified from oligodT and random hexamer primed libraries. (c) Example tracks for read density along the gene length for oligodT and random hexamer primed libraries. (d) Poly(A) tail length Sm-associated mRNAs (CG3997, CG1349 and CG3776) and non-associated mRNA (RpS2) from Y12 IP in S2 cells. IN, input total RNA; IP, immunoprecipitated RNA. The labels denote the length of poly(A) tails. Oligo(dT)₂₀ was used as the reverse primer for the reverse transcription and subsequent PCR, therefore producing the ‘smear’ of poly(A) tail. See Figure S11 in Additional file 1 for analysis of poly(A) containing reads for selected Sm-associated mRNAs.