Figure 7

RNA-Sm association is cell type-specific and not due to re-assortment. (a) RIP-qRT-PCR in da-Gal4 VFP-SmD1 fly ovary (anti-GFP) and S2 cells (Y12). Negative controls (Ctrl) used are 5S rRNA, Act5C and Smt3. CG9042 (Gapdh) is used as the normalization standard. snRNAs are shown separately due to the difference in scale. (b) mRNAs associated with Sm proteins in ovaries but not in S2 cells are expressed in S2 cells. t-Test for significance between IP and Ctrl: *P < 0.05, **P < 0.01, ***P < 0.001. Error bars show standard deviation.