Figure 6

snRNPs associate with mature mRNAs in S2 cells. (a) Sm-associated mRNAs, as well as scaRNAs and snRNAs, can be pulled down by a TMG antibody in S2 cells. CG9042 (Gapdh) is used for normalization. (b) Enrichment analysis of the U1-70 K RIP-seq data in a volcano plot. The most highly enriched transcripts were labeled. The inset rectangular boxes highlight CG3776 and CG8108 mRNAs in the plot. Note: CG1349 and CG4692 could be associated with other snRNPs, and therefore not pulled down by U1-70 K. (c) CG8108 mRNA can be pulled down by TMG and Y12 antibodies in S2 cells. (d) CG8108 is expressed in similar levels in Drosophila ovary and S2 cells (data from FlyBase). (e) CG8108 mRNA is not enriched in ovary Sm RIP-seq. t-Test for significance between IP and control (Ctrl): *P < 0.05, **P < 0.01, ***P < 0.001). Error bars reflect the standard deviation.