Experimental workflow and quality control. (A) Cells were lysed and the complete cytosolic fraction was used for ribosomal fractionation. Pelleted nuclei and nine fractions (indicated A to I) derived from the ribosomal fractionation were subsequently used for RNA isolation and strand-specific RNA-seq. Fractions A1 and A2 as well as B1 and B2 were merged prior to the RNA-seq. (B) 2100 Bioanalyzer RNA 6000 Pico results showing the integrity of the collected RNA samples obtained by ribosomal fractionation. Each ribosomal fraction has an RNA integrity value of 10. These results also show the sample-specific content of tRNAs, 5S, 5.8S, 18S and 28S rRNA, which nicely indicate the purity of the fractionation. RIN, RNA integrity.