Comparisons of Smaug-bound, repressed and degraded data sets. (A) Smaug binding was assessed using the fold enrichment in Smaug RIPs compared to control RIPs and box plots were used to show the range of these enrichments for the targets of Smaug-mediated mRNA decay and for non-targets. (B) Smaug-mediated translational repression was assessed using the change in TI in smaug-mutant compared to wild type and box plots were used to show the range of these changes for the targets of Smaug-mediated mRNA decay and for non-targets. (C) Smaug binding and translational repression were quantified as described in (A) and (B), respectively, and these values were plotted against one another. The dashed vertical and horizontal lines represent the median values for Smaug binding and Smaug-mediated translational repression, respectively. (D) Venn diagrams to show the overlap between the genes whose mRNAs are bound by Smaug, those that are degraded by Smaug and those that are translationally repressed by Smaug (FDR <10%). Note that, for each comparison, only genes scored as ‘expressed’ in both data sets were included.