Smaug represses the translation of thousands of mRNAs in the early embryo. (A) The 500 bottom Smaug binders are the 500 genes whose mRNAs show the lowest fold enrichment in Smaug RIPs versus control RIPs and they were plotted as in Figure 4A. The solid diagonal line represents no enrichment and the dotted diagonal lines represent two-fold enrichment or depletion. (B) Genes were ranked based on the extent of the increase in TI in smaug mutant versus wild type, with the gene having the highest increase being ranked number one. Box plots were then used to show the range of ranks for all genes, and the bottom 250, 500 and 1,000 Smaug binders as defined in (A). (C) Kernel density plot showing the change in TI in smaug mutant versus wild type for the bottom 500 Smaug binders as defined in (A) compared to the top 500 Smaug binders and all genes in the data set.