Figure 7
Expression of normally spliced Senp2 cDNA partially restores survival of HnrpllthuT cells. CD8 T cells from Hnrpll+/+ or Hnrpllthu/thu OT-1 TCR-transgenic B6.SJL-CD45.1/CD45.2 mice were activated and spinoculated with defective retroviral particles containing either empty retroviral vector or the same vector bearing full length, normally spliced Senp2 cDNA, and injected into the circulation of normal C57BL/6 mice. (a) Blood from each recipient mouse was analyzed by flow cytometry 3 and 17 days later, enumerating the frequency of donor CD45.1+ T cells among CD8 cells at each timepoint and the percentage that survived between days 3 and 17. (b) Data from two independent experiments analyzed as in (a). (c) Staining of the donor-derived T cells for CD45RC: black and blue histograms, +/+ cells with empty or Senp2 vectors, respectively; red histogram, thu/thu cells with Senp2 vector.