RT-PCR validation of differential mRNA splicing of genes predicted by the Boolean scoring method. Genes with RNA-seq features associated with differential exon inclusion and intron retention were scored and ranked alongside a set of seven genes that we validated by RT-PCR to be differentially spliced. Fifteen genes that ranked by score among these validated cases were selected for further RT-PCR, of which eight also showed differential banding. These products have been separated by eletrophoresis on acrylamide gels. Alongside each gel picture for each gene is an ideogram of the inferred sequence of each product, estimated by band size and the expected products predicted by junction read abundance from RNA-seq data. A single replicate from each genotype only was appraised, and these correspond to wild-type (WT) replicate 2 and thunder replicate 2 in Figure 5.