Advancing the functional utility of PAR-CLIP by quantifying background binding to mRNAs and lncRNAs

Table 1 High-throughput sequencing detected reads from PAR-CLIP background samples are abundant

	Total reads (PARalyzer utilized)	Unique sites (Locations)
G20	38,644,636	706,883
G35	6,187,300	259,337
G45	7,539,669	379,718
HuR.rep1	9,525,625	2,411,483
HuR.rep2	32,111,810	4,024,972
Total	35,924,998	2,857,291

Total PARalyzer Utilized Reads from three background samples were derived from the G20, G35 and G45 slices, two HuR replicates and a Total sample from 4SU-incorprated, UV 365 nm cross-linked and RNase T1 digested lysates that have been depleted for rRNA. Unique Locations are genomic sites derived after accounting for overlapping reads.

ISSN: 1474-760X