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Figure 1 | Genome Biology

Figure 1

From: Advancing the functional utility of PAR-CLIP by quantifying background binding to mRNAs and lncRNAs

Figure 1

Control immunoprecipitations of PAR-CLIP gels show significant background RNA. Phosphorimages of SDS acrylamide gels following the PAR-CLIP protocol show radiolabeled RNPs from HEK293 cell lysates. The IP from each lysate contains four replicates. Brackets indicate extracted regions of the HuR RBP and controls using FLAG-GFP (tagged green fluorescent protein as G20, G35, G45) and no tagged protein.

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