Functional sensitivity of ZFP36 sites. (A) Mosaic plot of significantly differentially upregulated (blue) and downregulated genes (pink) with and without ZFP36 sites. Significantly enriched MSigDB canonical pathway gene sets for downregulated genes with ZFP36 sites (yellow outline). Cumulative distribution function plots of EGFP-ZFP36-induced changes in mRNA abundance for 3′ UTRs grouped (B) by number of ZFP36 sites and (C) by 3′ UTR length. For all correlation plots the color and shape of the node indicates specific data classes (circles: PAR-CLIP sites, squares: expression change upon RBP perturbation, grey diamonds: transcript features, blue: ZFP36-related feature, purple: ELAVL1-related feature). Edge line thicknesses are proportional to correlation coefficient strength and the direction of correlation is indicated by the sign and color (negative in red and positive in green). (D) Correlation (left) and partial correlation (right) analysis of number of ZFP36 sites per 3′ UTR (blue circles), EGFP-ZFP36-induced changes in mRNA abundance (blue squares) and 3′ UTR length for all genes. (E) Correlation (left) and partial correlation (right) analysis of the number of ZFP36 sites per mRNA region and EGFP-ZFP36-induced changes in mRNA abundance for all genes with at least one ZFP36 site. kb, kilobase; PAR-CLIP, photoactivatable ribonucleoside cross-linking and immunoprecipitation; TTP, tristetraprolin; UTR, untranslated region; FDR, false discovery rate; LFC, log2 fold change; OE, overexpression; CDS, coding sequence.