Figure 6

Bisulfite sequencing of the HS-40 enhancer region of the GFP transgene. (a) DNA methylation levels were reduced in Dnmt1^MommeD32 heterozygous mice compared to wild-type mice in adult spleens (n = 4 wild-type mice and n = 2 Dnmt1^MommeD32 mutant mice). Filled circles represent methylated CpG sites. (b) DNA methylation levels in adult spleens were unaffected in mice heterozygous for Wiz^MommeD30 and Rif^MommeD18 (n = 4 for Wiz^MommeD30 and n = 2 for Rif1^MommeD18). (c) DNA methylation levels in adult spleens of Rlf^MommeD8, Rlf^MommeD28 and Rlf^MommeD34 heterozygotes were increased compared to wild-type mice (n = 2 mice for all genotypes). (d) Rlf homozygotes showed significantly increased DNA methylation (T-test, P < 0.05) in adult male spleen (Rlf^MommeD8 and Rlf^MommeD34) and 16.5 dpc embryo (Rlf^MommeD28) compared to wild types (n = 3 for all genotypes).