Differential expression analysis using qRT-PCR validated gene set. (a) ROC analysis was performed using a qRT-PCR log2 expression change threshold of 0.5. The results show a slight advantage for DESeq and edgeR in detection accuracy. (b) At increasing log2 expression ratios (incremented by 0.1), representing a more stringent cutoff for differential expression, the performances of the Cuffdiff and limma methods gradually reduce whereas PoissonSeq performance increases. AUC, area under the curve.