Figure 7

Human PINT inhibits tumor cell growth. (A.B). Relative number of PINT-overexpressing HCT116 cells (PINT) or control cells (vector) that were (A) left untreated or (B) treated with 500 nM doxorubicin (DOX), as determined by MTS assay. (C) Relative number of cells of HCT116 stable cell lines in each phase of the cell cycle. Cells were treated as for (A) and (B) for 12 hours, and cell cycle phases were determined by fluorescence-activated cell sorting (FACS) of bromodeoxyuridine (BrdU) incorporation and propidium iodide (PI) staining. (D) Percentage of apoptotic cells in HCT116 stable cell lines treated as in (C), determined by quantification of annexin V-positive cells. (E,F). Relative number of PINT-overexpressing A549 cells (PINT) or control cells (vector) that were (E) left untreated or were treated with 500 nM doxorubicin (F), as determined by MTS assay. (G) Relative number of A549 stable cell lines in each phase of the cell cycle determined as in (C). (H) Relative number of cells of A549 stable cell lines undergoing apoptosis, determined as in (D). (I) Correlation of PINT with KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways. Significant correlation coefficients between the indicated cellular pathways and PINT microarray probes.