Figure 5From: Genome-wide analysis identifies a functional association of Tet1 and Polycomb repressive complex 2 in mouse embryonic stem cellsPRC2 interacts with Tet1 and is required for correct DNA hydroxymethylation. (A) Nuclear extracts of embryonic stem cells (ESCs) or mouse embryonic fibroblasts (MEFs) were immunoprecipitated with anti-IgG or anti-Tet1 antibodies. Western blotting analysis was performed using the antibodies indicated. For each input, 1% of nuclear extract was loaded. (B) Nuclear extracts of ESCs or MEFs were immunoprecipitated with anti-IgG or anti-Suz12 antibodies. Western blotting analysis was performed using the antibodies indicated. For each input, 2% of nuclear extract was loaded. (C) Western blotting analysis of extracts from the control (small hairpin green fluorescent protein; shGFP) or Suz12 knockdown (shSuz12) ESCs was performed using the antibodies indicated. (D) Dot-blot analysis and signal quantification of 5hmC and 5mC in DNA extracted from control or Suz12 knockdown ESCs. Single-stranded (ss)DNA was used as a loading control. The experiments were performed in triplicate.Back to article page