In vivo role of svbF7 motifs in Svb-dependent enhancers. Anti-LacZ staining (green) shows modifications of reporter gene expression resulting from individual and simultaneous inactivation of svbF7 motifs in (a) sha1, (b) nyo1, (c) tyn2, (d) sha3 and (e) dyl2 enhancers. Red boxes schematize evolutionarily conserved svbF7 motifs; the open black box, a site that does not appear conserved across Drosophilidae. (f-h) Quantification of residual activity following individual disruption of svbF7 sites. **P-value < 0.01; *P-value < 0.05. wt, wild type. Error bars represent the standart deviation.