Confirmation of transcript expression levels by Northern blot analysis. Northern blot analysis of samples from trophozoites, at 24 and 72 h post-encystation, and at 8 h post-excystation. Total RNA (20 µg) from each time point was used and expression levels of genes with regulated expression determined. For each probe, the gene being probed and its annotation are listed. A gene with no significant changes in gene expression (EIN_192230) was used as a loading control. The expression level (FPKM) at each time point included in the blot is shown for each gene.