Changes in cell morphology during encystation and excystation. Cell morphology at different time points during encystation and excystation. The top row shows representative phase contrast images; the bottom row shows cells stained with Syto-11 to stain the nuclei. Note that the numbers of nuclei vary between one and four per cell, indicating some heterogeneity among cells in the timing of events during encystation, with the majority of mature cysts with four nuclei. Scale bar indicates 10 µm. Total library size and Pearson correlation between biological replicates is shown for each time point.