Example of non-canonical major transcript common to all the 16 tissues analysed: AES (amino-terminal enhancer of split, ENSG00000104964). Read coverage for the gene (a) and screenshot from the Zmap manual annotation interface (b). UTR exons and splice variants with no annotated CDS are shown in red, coding exons are shown in green and the CDS portion of models annotated as NMD are shown in purple. Clusters containing >8,000 CAGE tags defining transcription start suites are shown as small blue boxes, CpG islands are shown as yellow boxes broken by horizontal red bars representing TSS predictions from EPONINE . The short horizontal green bars represent polyadenylation sites identified by polyAseq .