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Table 4 Germ-line transmission frequency of cyc and sqt nuclease-induced lesions in zebrafish

From: A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases

Targeting nuclease(s) Number of F0 screened Number of mutant F0s
sqt5TAL + sqt3TAL 56 6 (whole locus deletions)
sqt5TAL + sqtZFN2 28 2 (TSS deletions)
sqtZFN1 92 1 (4 bp insertion)
cyc5TAL + cyc3TAL 36 10 (9 founders with TSS deletions, and 1 with a non-TSS 151 bp deletion and a deletion + inversion + insertion)
  1. For each founder (F0), at least 30 F1 embryos for sqt, or 22 F1 embryos for cyc were analyzed.