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Table 3 Frequency of cyclopia and mid-line defects in cyc and sqt nuclease-injected embryos

From: A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases

Targeting nuclease(s) Wild type Cyclopia and midline defects Abnormal Dead Total (N)
6.25 pg cyc5TAL+cyc3TAL 176 (77.5%) 30 (13.2%) 8 (3.5%) 13 (5.8%) 227
12.5 pg cyc5TAL+cyc3TAL 134 (60.1%) 54 (24.2%) 17 (7.6%) 18 (8.1%) 223
25 pg cyc5TAL+cyc3TAL 11 (16.2%) 27 (39.7%) 27 (39.7%) 3 (4.4%) 68
25 pg sqtZFN2 62 (53.0%) 25 (21.4%) 18 (15.4%) 12 (10.2%) 117
50 pg sqtZFN2 32 (29.1%) 26 (23.6%) 37 (33.6%) 15 (13.6%) 110
25 pg sqt5TAL 22 (71.0%) 3 (9.7%) 0 (0.00%) 6 (19.3%) 31
50 pg sqt5TAL 43 (45.7%) 15 (16.0%) 18 (19.1%) 18 (19.1%) 94
25 pg sqt3TAL 18 (47.4%) 0 (0.00%) 5 (13.1%) 15 (39.5%) 38
50 pg sqt3TAL 29 (38.2%) 12 (15.8%) 15 (19.7%) 20 (26.3%) 76
25 pg sqt5TAL+sqtZFN2 22 (29.3%) 17 (22.7%) 19 (25.3%) 17 (22.7%) 75
25 pg sqt5TAL+sqt3TAL 30 (36.1%) 20 (24.1%) 15 (18.1%) 18 (21.7%) 83
  1. Numbers were tabulated from at least two independent experiments.