|
Targeting nuclease(s)
|
Wild type
|
Cyclopia and midline defects
|
Abnormal
|
Dead
|
Total (N)
|
|---|
|
6.25 pg cyc5TAL+cyc3TAL
|
176 (77.5%)
|
30 (13.2%)
|
8 (3.5%)
|
13 (5.8%)
|
227
|
|
12.5 pg cyc5TAL+cyc3TAL
|
134 (60.1%)
|
54 (24.2%)
|
17 (7.6%)
|
18 (8.1%)
|
223
|
|
25 pg cyc5TAL+cyc3TAL
|
11 (16.2%)
|
27 (39.7%)
|
27 (39.7%)
|
3 (4.4%)
|
68
|
|
25 pg sqtZFN2
|
62 (53.0%)
|
25 (21.4%)
|
18 (15.4%)
|
12 (10.2%)
|
117
|
|
50 pg sqtZFN2
|
32 (29.1%)
|
26 (23.6%)
|
37 (33.6%)
|
15 (13.6%)
|
110
|
|
25 pg sqt5TAL
|
22 (71.0%)
|
3 (9.7%)
|
0 (0.00%)
|
6 (19.3%)
|
31
|
|
50 pg sqt5TAL
|
43 (45.7%)
|
15 (16.0%)
|
18 (19.1%)
|
18 (19.1%)
|
94
|
|
25 pg sqt3TAL
|
18 (47.4%)
|
0 (0.00%)
|
5 (13.1%)
|
15 (39.5%)
|
38
|
|
50 pg sqt3TAL
|
29 (38.2%)
|
12 (15.8%)
|
15 (19.7%)
|
20 (26.3%)
|
76
|
|
25 pg sqt5TAL+sqtZFN2
|
22 (29.3%)
|
17 (22.7%)
|
19 (25.3%)
|
17 (22.7%)
|
75
|
|
25 pg sqt5TAL+sqt3TAL
|
30 (36.1%)
|
20 (24.1%)
|
15 (18.1%)
|
18 (21.7%)
|
83
|
- Numbers were tabulated from at least two independent experiments.
