Table 2 Frequency of deformities and lethality in egfp TAL-injected embryos
Targeting nuclease(s) | Wild type | Abnormal | Dead | Total (N) |
|---|---|---|---|---|
12.5 pg egfp5TAL | 86 (93.5%) | 6 (6.5%) | 0 (0%) | 92 |
25 pg egfp5TAL | 14 (23.0%) | 44 (72%) | 3 (4.9) | 61 |
12.5 pg egfp3TAL | 89 (96.7%) | 2 (2.2%) | 1 (1.1%) | 92 |
25 pg egfp3TAL | 53 (61.6%) | 28 (32.6%) | 5 (58.0%) | 86 |
12.5 pg egfp5TAL+egfp3TAL | 202 (82.8%) | 29 (11.9%) | 13 (5.3%) | 244 |
25 pg egfp5TAL+egfp3TAL | 40 (33.1%) | 62 (51.2%) | 19 (15.7%) | 121 |