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Table 1 The T. urartu and T. turgidum final assembly statistics

From: Separating homeologs by phasing in the tetraploid wheat transcriptome

  T.urartu T.turgidum
100-bp paired-end reads (n) 248.5 million 488.9 million
Reads after digital normalizationa (n) 47.3 million 110.7 million
Contigs (n) 86,247 140,118
Mean contig size (bp) 1,417 bp 1,299 bp
Min contig size (bp) 212 bp 298 bp
Max contig size (bp) 17,959 bp 26,226 bp
GC content (%) 49% 49%
Total transcriptome size (Mb) 122 Mb 181 Mb
Reads mapping to the assembly (% of total reads) 82.2% 81.5%
Reads mapped in proper pairs (% of total reads) 73.0% 71.5%
Unique alignments (% of total mapped) 52.8% 76.7%
Benchmark genesb assembled > 50% length in a single contig 12,693 (94%) 12,961 (96%)
Benchmark genesb assembled > 90% length in a single contig 10,727 (80%) 10,197 (76%)
  1. aElimination of Homo sapiens,Escherichia coli, wheat mitochondrial, rRNA, and chloroplast sequences resulted in the elimination of 0.5% of the digitally normalized reads in T. urartu and 0.6% in T. turgidum.
  2. b13,472 full-length cDNAs from the RIKEN Plant Science Center Japan [35].