Comparison of the effect of different k-mers on transcriptome assembly metrics in diploid and tetraploid wheat. T. urartu values are indicated by the red dotted lineand T. turgidum by theblue solid line. (A) Average contigs length. (B) Total number of contigs. (C) Percent of total reads mapped back to the assembly. (D) Percent of total reads that are mapped in proper pairs. (E) Fraction of 13,472 full-length benchmark wheat cDNAs that are assembled in a single contig. (F) Venn diagram showing the number of benchmark cDNAs assembled full-length (>90%) at k-mer sizes 21 and 63.