Analyzing the soybean transcriptome during autoregulation of mycorrhization identifies the transcription factors GmNF-YA1a/b as positive regulators of arbuscular mycorrhization

Table 2 Transcript analysis of NF-YA and phosphate transporter genes in A. rhizogenes-transformed roots

	EV			RNAi			Ratio
Gene	Mean		SD	Mean		SD	(RNAi/EV)
Glyma03g36140 (GmNF-YA1a)	0.21	±	0.097	0.084	±	0.048	0.40***
Glyma19g38800 (GmNF-YA1b)	0.19	±	0.099	0.082	±	0.055	0.44***
Glyma10g10240 (putative NF-YA)	0.021	±	0.014	0.017	±	0.015	0.80
Glyma02g35190 (putative NF-YA)	0.036	±	0.018	0.023	±	0.015	0.65**
Glyma13g08720 (phosphate transporter)	0.026	±	0.018	0.0098	±	0.0075	0.38***
Glyma14g28780 (phosphate transporter)	0.15	±	0.10	0.060	±	0.051	0.40***
Glyma14g36650 (phosphate transporter)	0.0026	±	0.0023	0.0010	±	0.0006	0.37***
RiBTub1	0.0036	±	0.0028	0.0014	±	0.0011	0.40***

Transcript levels of different NF-YA genes and AM-induced phosphate transporters were exemplified analyzed in chimeric plants with a Bragg wild-type background. Transcripts relative to GmSUBI-1 were determined by RT-qPCR using gene-specific primers (for primer information see Table S4 in Additional file 1). Specificity of primer pairs was assessed by sequencing of RT-qPCR products. Data are mean values + SD with n = 30 and n = 35 for EV and RNAi, respectively, coming from two independent experiments. Data of empty vector control and RNAi plants were pairwise compared by the Student's t-test. *P ≤0.05, **P ≤0.01, ***P ≤0.001. Significant changes are highlighted in bold.
EV: empty vector control; RNAi: roots expressing the RNAi construct.

ISSN: 1474-760X