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Table 1 Suggestions for the design of mapping-by-sequencing experiments

From: User guide for mapping-by-sequencing in Arabidopsis

  Outcross populations Backcross populations Direct sequencing Deep candidate resequencing (dCARE)
Generation F2 BC1F2 BC1-3F2a n/a
Mutants (n) Approximately 150 Approximately 50 1 As many as possible
Optimal coverage >25 Approximately 50 >25 n/a
Sequencing type Paired-end Paired-end Paired-end Single-end
  1. aDepending on mutation rate.