Table 1 Suggestions for the design of mapping-by-sequencing experiments
Outcross populations | Backcross populations | Direct sequencing | Deep candidate resequencing (dCARE) | |
|---|---|---|---|---|
Generation | F2 | BC1F2 | BC1-3F2a | n/a |
Mutants (n) | Approximately 150 | Approximately 50 | 1 | As many as possible |
Optimal coverage | >25 | Approximately 50 | >25 | n/a |
Sequencing type | Paired-end | Paired-end | Paired-end | Single-end |